The Empress (1503-1539) wears a luxurious ornamental skirt with grutesco decorations in fashion during the fifteen thirties.

Carlos V commissioned Leone Leoni to make this statue along with other portraits of the Hapsburgs. It was carried out years after Isabel's death, and the artist based it on certain portraits by Titian (P415, for example). Stylistically, it is inspired by the statue of Margarita de Austria in the Mausoleum of Maximilian I at Innsbruck.

Years after it was made, it was completed by the silversmith, Felipe Jusarte, who handled the details of the dress, while the sculptor, Micale Méndez gave it its finish.