Joanna, the daughter of Charles V and Isabella of Portugal was born in 1535, and later married her cousin Joao Manuel, prince of Brazil. On the death of her husband in 1554, and having soon after given birth to their son, Don Sebastián, she left Portugal and returned to Spain, where she died in 1573.
A fire in El Pardo Palace on March 13, 1604, destroyed the portraits in the Hall of Kings. These portraits had been painted by the finest artists of the time, including Titian, Antonio Moro, Alonso Sánchez Coello, and Sofonisba Anguisciola. Encased in stucco frames attached to the walls of the gallery, which had been organized by Sánchez Coello at the behest of Philip II, these portr